Phytochemical Analysis, Antibacterial and Antibiofilm Activities of Aloe vera Aqueous Extract against Selected Resistant Gram-Negative Bacteria Involved in Urinary Tract Infections

نویسندگان

چکیده

In bacterial infections, including urinary tract infections (UTIs), the gap between development of new antimicrobials and antimicrobial resistance is dramatically increasing, especially in Gram-negative (Gram–) bacteria. All healthy products that can be used per se or may sources antibacterial compounds should considered fight against this major public health threat. present study, phytochemical composition Aloe vera extract was investigated by HPLC–MS/MS, we further evaluated its antibiofilm formation activity selected resistant Gram– bacteria involved UTIs, namely, Achromobacter xylosoxidans 4892, Citrobacter freundii 426, Escherichia coli 1449, Klebsiella oxytoca 3003, Moraxella catarrhalis 4222, Morganella morganii 1543, Pseudomonas aeruginosa 3057, a reference strain E. ATCC 25922. Inhibition zones (IZs) were determined using well diffusion method, minimum inhibitory (MIC), bactericidal (MBC) concentration two-fold serial microdilution assay, crystal violet attachment assay. Aloe-emodin derivatives constituent (75.74%) A. extract, most important them being aloesin (30.22%), aloe-emodin-diglucoside (12.58%), 2?-p-methoxycoumaroylaloeresin B (9.64%). The minerals found sulfur (S), silicon (Si), chlorine (Cl), potassium (K), bromine (Br). Except for clinical which totally non-susceptible, demonstrated noteworthy with MIC MBC values ranging from 0.625 to 5 mg/mL 10 mg/mL, respectively. also dose-dependent effects, 25922 susceptible = IZ 19 mm at 20 mg/mL. potential strong 2MIC (93–100% biofilm inhibition), moderate MIC/2 (32–41%), weak MIC/4 (14–21%), nil MIC/8.

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ژورنال

عنوان ژورنال: Fermentation

سال: 2022

ISSN: ['2311-5637']

DOI: https://doi.org/10.3390/fermentation8110626